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KMID : 0545120120220081035
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 8 p.1035 ~ p.1043
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Comparison of Ammonia-Oxidizing Bacterial Community Structure in Membrane-Assisted Bioreactors Using PCR-DGGE and FISH
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A. Ziembinska
S. Ciesielski
A. Gnida
S. Zabczynski
J. Surmacz-Gorska
K. Miksch
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Abstract
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The ammonia-oxidizing bacterial (AOB) communities in three membrane bioreactors (MBRs) were monitored for 2 months after an acclimation period in order to investigate the influence of sludge age and medium type on AOB changeability and its connection with nitrification effectiveness. One MBR with a sludge age of 4 days was fed with a synthetic medium, whereas the other two with sludge ages of 8 and 32 days were fed with landfill leachate. The research revealed that landfill leachate can be effectively treated in an MBR with a higher sludge age for longer periods of time and that this improvement in performance was correlated with an increase in AOB biodiversity. Interestingly, the medium type has a stronger influence on AOB biocenosis formation than the sludge age.
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KEYWORD
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activated sludge, ammonia oxidizing bacteria, PCR-DGGE, FISH, MBR, 16S rRNA gene
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